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Ubiquitin-conjugating Enzyme E2 F; NCE2; NEDD8-conjugating Enzyme UBE2F; NEDD8 Protein Ligase UBE2F

Protein

E. coli

Human UBE2F (Accession Nr. NP_542409) fused to a N-terminal His-tag.

Human

Liquid. In 50mM HEPES pH 7.5, 200mM NaCl, 10% Glycerol (v/v), 1mM TCEP.

Use: Member of the NEDD8-conjugating (E2) enzyme family that receives NEDD8 from a NEDD8-activating (E1) enzyme and subsequently interacts with a NEDD8 ligase (E3) to conjugate NEDD8 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1-­1μM.

Manufactured by Boston Biochem

DRY ICE

-20°C

-80°C

Aliquot to avoid freeze/thaw cycles.

Stable for at least 1 year after receipt when stored at -80°C.

No

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Ubiquitin-conjugating Enzyme E2F (UBE2F) is a NEDD8-conjugating enzyme (E2). It is a 185 amino acid (aa) protein with a predicted molecular weight of 21 kDa. The human protein exhibits approximately 96% aa sequence identity with its mouse and rat orthologs. UBE2F is involved the covalent attachment of NEDD8 to target proteins in a process termed neddylation. For example, UBE2F appears to participate in the neddyylation of Cullin 5. Members of the Cullin family (Cullin 1-7) are scaffold proteins that can incorporate into multimolecular complexes termed Cullin RING ubiquitin Ligases (CRLs). CRLs make up the largest subset of ubiquitin ligases (E3), and their activity is promoted by neddylation of associated Cullin family proteins.