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Protein

E. coli

Human Ubc13/Uev1a Complex (Accession Nr. Ubc13 | Uev1a) fused to a N-terminal His-tag.

Human

Liquid. In 50mM HEPES pH 7.5, 200mM NaCl, 10% Glycerol (v/v), 2mM TCEP.

Use: Member of the ubiquitin­conjugating (E2) enzyme family that receives ubiquitin from a ubiquitin­activating (E1) enzyme and subsequently interacts with a ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1-­1μM.

Manufactured by Boston Biochem

DRY ICE

-20°C

-80°C

Aliquot to avoid freeze/thaw cycles.

Stable for at least 1 year after receipt when stored at -80°C.

No

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Ubiquitin-conjugating Enzyme E2N (UBE2N), also known as ubiquitin-conjugating enzyme 13 (Ubc13), forms a functional complex with the catalytically inactive Uev1a/UBE2V1 protein. Human UBE2N/Ubc13 shares 100% and 99% amino acid (aa) sequence identity with the mouse and rat orthologs, respectively. Human Uev1a/UBE2V1 shares 89% aa sequence identity with its mouse and rat orthologs. The UBE2N (Ubc13)/Uev1a complex functions with ubiquitin ligases (E3s), including TRAF-6 and TRIM5, to synthesize Lys63-linked ubiquitin chains. These ubiquitin chains can either be unanchored or attached to target proteins. The UBE2N (Ubc13)/Uev1a complex localizes primarily to the cytoplasm and is important for NF-κB activation. Additionally, this complex may function in the intracellular response to retroviral capsid recognition. Pathologically, the UBE2N (Ubc13)/Uev1a complex is required for the proliferation of diffuse large B-cell lymphoma cells, suggesting that it may play a role in cancer.