Recombinant Human PA28 Activator alpha Subunit Protein, CF Summary
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-381
| Formulation | X mg/ml (X µM) in 50 mM Hepes, pH 8.0, 200 mM NaCl, 1mM DTT. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: PA28 Activator alpha Subunit
The Proteasome Activator 28 (PA28) Regulatory Complex, also known as the 11S Regulatory (REG) Complex, is a ring-shaped, multimeric, ATP-independent regulatory complex that can bind to one or both ends of the 20S Proteasome or 20S Immunoproteasome (1,2). The PA28 Regulatory Complex stimulates the proteasome to hydrolyze small peptides, but not ubiquitinated proteins, for the production of peptides for MHC class I antigen presentation (3-7). Three PA28 Regulatory Complex subunits, alpha, beta, and gamma, have been identified and can oligomerize to form the PA28 Regulatory Complex (8-10). While the expression of the PA28alpha and beta subunits are induced by IFN-gamma, the PA28gamma subunit is not significantly affected (11,12). In vivo, PA28 alpha forms hetero-heptamers or -hexamers with PA28 beta(13-15). PA28 alpha can also form active homo-heptamers in vitro and can be used to activate the 20S Proteasome (3,16). In addition to regulating production of MHC class I antigens, PA28 alpha may protect against oxidative stress (17).
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- Kuehn, L. & B. Dahlmann (1997) Mol. Biol. Rep. 24:89.
- Li, J. & M. Rechsteiner (2001) Biochimie 83:373.
- Realini, C. et al. (1997) J. Biol. Chem. 272:25483.
- Jiang, H. & J.J. Monaco (1997) Immunogenetics 46:93.
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- Zhang, Z. et al. (1999) Biochemistry 38:5651.
- Song, X. et al. (1996) J. Biol. Chem. 271:26410.
- Mott, J.D. et al. (1994) J. Biol. Chem. 269:31466.
- Johnston, S.C. et al. (1997) Protein Sci. 6:2469.
- Li, J. et al. (2011) FASEB J. 25:883.
Citation for Recombinant Human PA28 Activator alpha Subunit Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
1Citation: Showing 1 - 1
- Structure- and function-based design of Plasmodium-selective proteasomeinhibitors.Authors: Li H, O"Donoghue A, van der Linden W, Xie S, Yoo E, Foe I, Tilley L, Craik C, da Fonseca P, Bogyo MNature, 2015;530(7589):233-6.Species: HumanSample Types: ProteinApplications: Enzyme Assay
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