S5a/Angiocidin, also known as Anti-secretory Factor (ASF), is classified under the gene PSMD4, but is often referred to by a different name depending on the context in which it is described. S5a and ASF have identical 377 amino acid (aa) sequences, while Angiocidin is described as having an additional Gly255Glu256Arg257 sequence in its C-Terminus (1-3). The human protein shares 96% and 99% aa sequence identity with its mouse and rat orthologs, respectively. Structurally, it contains an N-terminal von Willebrand Factor type A domain and two C-terminal Ubiquitin-interacting motifs (UIM). It acts as a Ubiquitin-binding protein where it is most commonly referred to as S5a or in yeast as Rpn10. It is part of the 19S regulatory subunit of the 26S Proteasome where its UIM recognizes poly-ubiquitinated proteins destined for degradation (4). As a part of the proteasome complex, it may also recognize the Ubiquitin-like modifier FAT10 (5). Free cytoplasmic forms also exist where its ubiquitination is catalyzed by a range of Ubiquitin E3 ligases from different classes. Therefore, experimentally S5a/Angiocidin may act as a useful substrate to monitor the activity of (E3) ligases, independent of their specific mechanisms of action (6). In cancer biology, where it is often referred to as Angiocidin, it is shown to slow tumor progression. It is found in the extracellular matrix of certain tumor subtypes, and it may act by suppressing angiogenesis or by directly inhibiting tumor cell growth (7,8). It also is found in several biological fluids where it is known primarily as ASF. It suppresses fluid secretion in response to enterotoxin and may act as an anti-inflammatory factor (9-11).
S5a/Angiocidin has a low affinity for mono-, di-, and tri-Ubiquitin but binds efficiently to tetrameric ubiquitin and has a preference for longer Ubiquitin polymers. This affinity resin can be used for the enrichment, isolation and identification of ubiquitinated proteins, 26S substrates or proteins that contain ubiquitin-like domains.
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsThere are currently no reviews for this product. Be the first toreview Recombinant Human S5a/Angiocidin Agarose Protein, CF and earn rewards!
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Boston Biochem是泛素相关研究产品的全球领先生产商。自1997年以来,我们的使命一直是为泛素蛋白酶体途径(UPP)研究人员提供创新工具,以促进并加速药物发现的努力。波士顿生物化学专门从事泛素和类泛素(UBL)蛋白(例如Apg8,FAT10,ISG15,NEDD8,SUMO,UFM1)以及相关酶,底物,抑制剂和试剂盒的几种相关产品系列。