AQUApure Tetra-Ub Chains (K63-linked) Protein, CF Summary
Ub-AQUA analysis:
K63: 99.04%
K11: 0.76%
K6 : 0.13%
All other linkages ≤ 0.04%
Ubiquitin chains vary in length, linkage, and function. K63-linked Tetra-Ubiquitin Chains (Ub4) are ideal for investigating Ubiquitin-binding proteins and as substrates for Ubiquitin-specific isopeptidases. Reaction conditions will need to be optimized for each specific application. IMPORTANT: Heating this product in SDS-PAGE buffer or terminating reactions containing this product with heated SDS-PAGE buffer could lead to unexpected, high apparent molecular weight banding or smearing on gels that is not representative of product purity. For optimal results, we recommend incubation in SDS-PAGE buffer + DTT at <40 °c="" for="" 20="" minutes="" prior="" to="" gel="">
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
UC-310B
| Formulation | 1 mg/ml (29 μM) in sterile, deionized water |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Tetra-Ubiquitin
Linkage specific Poly-Ubiquitin chains may be used as a substrate for in vitro reactions with deubiquitinating enzymes ("DUB"s") that cleave the peptide or isopeptide linkage between adjacent Ubiquitin molecules. Poly-Ubiquitin chains can also be used to investigate mechanisms of binding and recognition between the chains and other proteins that contain Ubiquitin-Associated domains (UBAs), Ubiquitin-interacting motifs (UIMs), ZnF"s and/or other Ubiquitin-sensing elements.
K63-linked Tetra-Ubiquitin chains are manufactured using recombinant Ubiquitin and purely enzymatic techniques to avoid the potential for contaminating synthetic intermediates. The correctness of linkage and purity of each production lot is assessed using the Absolute Quantitation of Ubiquitin method (Ub-AQUA), an LCMS-based technique that provides extremely accurate information on the composition of Poly-Ubiquitin samples.
- Kirkpatrick D.S. et al. (2006) Nat Cell Biol. 8: 700-10
- Ordureau, A. et al. (2014) Mol. Cell 56: 360–375
- Ordureau, A. et al. (2015) Pro. Nat. Acad. of Sci. USA 112: 6637–6642
- Phu L. et al. (2011) Mol Cell Proteomics 10: M110.003756
- Wall C.E. et al. (2019) Cell Reports 29: 3280
Citations for AQUApure Tetra-Ub Chains (K63-linked) Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
8Citations: Showing 1 - 8Filter your results:
Filter by:
- The ubiquitin interacting motifs of USP37 act on the proximal Ub of a di-Ub chain to enhance catalytic efficiencyAuthors: N Manczyk, G Veggiani, J Teyra, AW Strilchuk, SS Sidhu, F SicheriSci Rep, 2019;9(1):4119.Applications: Bioassay
- USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilizationAuthors: S Yang, L Liu, C Cao, N Song, Y Wang, S Ma, Q Zhang, N Yu, X Ding, F Yang, S Tian, K Zhang, T Sun, J Yang, Z Yao, S Wu, L ShiNat Commun, 2018;9(1):1285.Applications: Bioassay
- Regulation of HLTF-mediated PCNA polyubiquitination by RFC and PCNA monoubiquitination levels determines choice of damage tolerance pathwayAuthors: Y Masuda, S Mitsuyuki, R Kanao, A Hishiki, H Hashimoto, C MasutaniNucleic Acids Res., 2018;0(0):.Species: N/ASample Types: Recombinant ProteinApplications: Standard
- ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote Genome StabilityAuthors: P Haahr, N Borgermann, X Guo, D Typas, D Achuthanku, S Hoffmann, R Shearer, TK Sixma, N MailandMol. Cell, 2018;0(0):.Species: HumanSample Types: Cell LysatesApplications: Bioassay
- OTUD4 Is a Phospho-Activated K63 Deubiquitinase that Regulates MyD88-Dependent SignalingAuthors: Y Zhao, MC Mudge, JM Soll, RB Rodrigues, AK Byrum, EA Schwarzkop, TR Bradstreet, SP Gygi, BT Edelson, N MosammaparMol. Cell, 2018;69(3):505-516.e5.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
- Novel polyubiquitin imaging system, PolyUb-FC, reveals that K33-linked polyubiquitin is recruited by SQSTM1/p62Authors: Y Nibe, S Oshima, M Kobayashi, C Maeyashiki, Y Matsuzawa, K Otsubo, H Matsuda, E Aonuma, Y Nemoto, T Nagaishi, R Okamoto, K Tsuchiya, T Nakamura, S Nakada, M WatanabeAutophagy, 2017;0(0):1-43.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
- Tandem UIMs confer Lys48 ubiquitin chain substrate preference to deubiquitinase USP25Authors: K Kawaguchi, K Uo, T Tanaka, M KomadaSci Rep, 2017;7(0):45037.Species: N/ASample Types: ProteinApplications: Bioassay
- Non-canonical regulation of SPL transcription factors by a human OTUB1-like deubiquitinase defines a new plant type rice associated with higher grain yieldAuthors: S Wang, K Wu, Q Qian, Q Liu, Q Li, Y Pan, Y Ye, X Liu, J Wang, J Zhang, S Li, Y Wu, X FuCell Res., 2017;27(9):1142-1156.Species: Plant - Oryza sativa (Rice)Sample Types: Recombinant ProteinApplications: Bioassay
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for AQUApure Tetra-Ub Chains (K63-linked) Protein, CF
There are currently no reviews for this product. Be the first toreview AQUApure Tetra-Ub Chains (K63-linked) Protein, CF and earn rewards!
Have you used AQUApure Tetra-Ub Chains (K63-linked) Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Boston Biochem是泛素相关研究产品的全球领先生产商。自1997年以来，我们的使命一直是为泛素蛋白酶体途径（UPP）研究人员提供创新工具，以促进并加速药物发现的努力。波士顿生物化学专门从事泛素和类泛素（UBL）蛋白（例如Apg8，FAT10，ISG15，NEDD8，SUMO，UFM1）以及相关酶，底物，抑制剂和试剂盒的几种相关产品系列。
暂无问答
本网站将在规定时间内给予删除等相关处理。
