Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

Data Examples

Western BlotView Larger

Detection of Human SUMO2/3 by Western Blot.Western blot shows lysates of SUMO2/3. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human SUMO2/3 Monoclonal Antibody (Catalog # A-718) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SUMO2/3 at approximately 16-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western BlotView Larger

Detection of Human SUMO2/3 by Western Blot.Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human SUMO2/3 Monoclonal Antibody (Catalog # A-718) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for SUMO2/3 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Background: SUMO2/3

Small Ubiquitin­like Modifiers (SUMOs) are a family of small, related proteins that can be enzymatically attached to a target protein by a post­translational modification process termed SUMOylation. Unlike ubiquitination, which targets proteins for degradation, SUMOylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. All human SUMO proteins share a conserved ubiquitin-like domain and a C-terminal diglycine cleavage/attachment site. Human SUMO1, SUMO2, SUMO3, and SUMO4 are all translated as propeptides, containing C-terminal prosegments following the diglycine motif that marks the end of the mature forms. Following prosegment cleavage, SUMO1, 2, and 3 may then be enzymatically attached to a lysine on a target protein. It is not clear whether SUMO4 is processed in a similar fashion.

Product Datasheets

Related Research Areas

• Ubiquitin-like Modifiers (UBLs) and Regulators